منابع مشابه
Identification of the Hfq-binding site on DsrA RNA: Hfq binds without altering DsrA secondary structure.
DsrA RNA regulates the translation of two global regulatory proteins in Escherichia coli. DsrA activates the translation of RpoS while repressing the translation of H-NS. The RNA-binding protein Hfq is necessary for DsrA to function in vivo. Although Hfq binds to DsrA in vitro, the role of Hfq in DsrA-mediated regulation is not known. One hypothesis was that Hfq acts as an RNA chaperone by unfo...
متن کاملHfq variant with altered RNA binding functions
The interaction between Hfq and RNA is central to multiple regulatory processes. Using site-directed mutagenesis, we have found a missense mutation in Hfq (V43R) which strongly affects2 the RNA binding capacity of the Hfq protein and its ability to stimulate poly(A) tail elongation by poly(A)-polymerase in vitro. In vivo, overexpression of this Hfq variant fails to stimulate rpoS-lacZ expressio...
متن کاملHfq proximity and orientation controls RNA annealing
Regulation of bacterial gene networks by small non-coding RNAs (sRNAs) requires base pairing with messenger RNA (mRNA) targets, which is facilitated by Hfq protein. Hfq is recruited to sRNAs and mRNAs through U-rich- and A-rich-binding sites, respectively, but their distance from the sRNA-mRNA complementary region varies widely among different genes. To determine whether distance and binding or...
متن کاملOn the facultative requirement of the bacterial RNA chaperone, Hfq.
The pleiotropic post-transcriptional regulator Hfq is an RNA chaperone that facilitates pairing interactions between small regulatory RNAs (sRNAs) and their mRNA targets in several bacteria. However, this classical pattern, derived from the Escherichia coli model, is not applicable to the whole bacterial kingdom. In this article we discuss the facultative requirement for Hfq for sRNA-mRNA duple...
متن کاملReflections on 20 years of RNA.
My introduction to the RNA world started approximately 20 years before the 1994 founding of this journal; a few remarks on how I happened to take up RNA as a research theme during that time will serve as background to the excitement and promise for RNA studies that I sensed ca. 1994. As an undergraduate biochemistry major I had enjoyed enzymology and became intrigued by the ribosome, but though...
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ژورنال
عنوان ژورنال: RNA
سال: 2015
ISSN: 1355-8382,1469-9001
DOI: 10.1261/rna.050047.115